Apotome 2 Zeiss - gainseattle.com

ZEISS ApoTome.2 - Mouse Embryo Optical.

Create optical sections of your fluorescent samples – free of scattered light. With structured illumination, you know that only the focal plane appears in your image: Apotome.2 recognizes the magnification and moves the appropriate grid into the beampath. 21/11/2011 · When you observe fluorescence-labeled specimens in 3D, specimen details outside the depth-of-field of the objective appear blurred. This reduces the signal-to-noise ratio in your data. ApoTome.2 solves this problem by. 21/08/2012 · Optical sectioning right comparison with widefield microscopy left. Mouse embryo, tissue section, green: GFP, red: Cy3. Objective: Plan APOCHROMAT 40 x/1.3 Oil. 21/08/2012 · Rat hippocampus, triple fluorescence, maximum projection of 3D image stack. Objective: Plan-APOCHROMAT 63x/1.4. Courtesy of E. Fuchs & S. Bauch, DPZ Göttinge.

ApoTome.2 Notes on device safety Carl Zeiss 05/2012 423667-7144-001 9 Dust and dirt can impair the performance of the instrument. Therefore, protect the instrument from these influences as far as possible and cover it with the dust cover when not in use. Microscópio Axio Imager com objetivas de 10, 20, 40 e 60X para aquisição de imagens de luz transmitida por D.I.C, sistema de epi-iluminação HXP e módulo Apotome 2 para aquisição de imagens confocais. Estão disponíveis filtros para DAPI, GPF/FITC e Rodamina. The tutorial initializes with an animation of the ZEISS ApoTome in action featured in the upper left-hand side of the window. Beneath the ApoTome model is a cutaway view of the light path showing the aperture grid and the rotating optical glass plate that directs a projection of the grid across the specimen. Learn how to make use of ZEISS ApoTome and discover the concept of structured illumination thanks to this tutorial. You will then be able to image even thick samples very clearly. The raw image set gathered by the ZEISS ApoTome is treated with a simple algebraic function by the application software to produce a single sharp, crisp derivative image that is free of the blur arising from remote focal planes.

Choose your system out of six different stand versions and combine with high end fluorescence imaging systems: ApoTome.2 and laser scanning microscopes like LSM 900 and LSM 980. Whether you are simply observing and recording, or performing highly complex imaging experiments: it’s easy to adapt the system components to the application at hand. ApoTome Operation The basic concept behind the ZEISS ApoTome is the use of an evenly spaced grid in the aperture plane to serve as a mask through which the specimen is illuminated. The grid is inserted into the light path of the microscope and uses the epi-illuminator lens system to project a shadow of the grid lines into sharp focus, superimposed on the specimen, in the objective focal plane.

Apotome.2. Optical sectioning using structured illumination for brilliantly resolved 2D or 3D fluorescence images free from stray light - now includes Apotome deconvolution algorithm.

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